2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Substantiae normales in curva calibrationis distributionis massae molecularis relativae adhibitae: insulinum, mycopeptida, glycina-glycina-tyrosinum-argininum, glycina-glycina-glycina
3 Instrumenta et apparatum
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Summa summarum, proportio aminoacidorum in productis Sustar maior est quam in productis Zinpro.
Pars VIII Effectus usus
Effectus variarum fontium mineralium vestigialium in productionem et qualitatem ovorum gallinarum ovipararum in periodo oviparae serotinae.
Processus Productionis
Technologia chelationis directae
Technologia emulsificationis tonsionis
Technologia pulverisationis sub pressione et siccationis
Technologia refrigerationis et dehumidificationis
Technologia provecta moderationis environmentalis
Appendix A: Methodi ad distributionem massae molecularis relativae peptidorum determinandam
Adoptio normae: GB/T 22492-2008
1 Principium Probationis:
Determinatum est per chromatographiam filtrationis gel altae efficaciae. Hoc est, adhibito impletione porosa ut phase stationaria, fretus differentia in magnitudine massae molecularis relativae partium exemplaris ad separationem, detecta ad vinculum peptidicum longitudinis undae absorptionis ultraviolaceae 220nm, utens programmate dedicato ad determinationem distributionis massae molecularis relativae per chromatographiam filtrationis gel (id est, programmate GPC), chromatogrammata et eorum data processa sunt, computataque ad magnitudinem massae molecularis relativae peptidi soiae et ambitum distributionis obtinendum.
2. Reagentia
Aqua experimentalis specificationi aquae secundariae in GB/T6682 satisfacere debet; usus reagentium, exceptis provisionibus specialibus, analytice purus est.
2.1 Reagentia includunt acetonitrilum (chromatographice purum), acidum trifluoroaceticum (chromatographice purum),
2.2 Substantiae normales in curva calibrationis distributionis massae molecularis relativae adhibitae: insulinum, mycopeptida, glycina-glycina-tyrosinum-argininum, glycina-glycina-glycina
3 Instrumenta et apparatum
3.1 Chromatographum Liquidum Altae Perfunctionis (HPLC): statio laboris vel integrator chromatographicus cum detectore UV et programmate GPC ad notitias tractandas.
3.2 Unitas filtrationis et degassationis vacui phasis mobilis.
3.3 Libra electronica: valor graduatus 0.000 1g.
Quattuor gradus operandi
4.1 Conditiones chromatographicae et experimenta adaptationis systematis (conditiones referentiales)
- 4.1.1 Columna chromatographica: TSKgelG2000swxl300 mm × 7.8 mm (diametro interiore) vel aliae columnae gel eiusdem generis cum simili effectu ad determinationem proteinorum et peptidorum aptae.
- 4.1.2 Phasis mobilis: Acetonitrilum + aqua + acidum trifluoroaceticum = 20 + 80 + 0.1.
- 4.1.3 Longitudo undae detectionis: 220 nm.
- 4.1.4 Fluxus celeritas: 0.5 mL/min.
- 4.1.5 Tempus detectionis: 30 min.
- 4.1.6 Volumen iniectionis speciminis: 20μL.
- 4.1.7 Temperatura columnae: temperatura ambiente.
- 4.1.8 Ut systema chromatographicum requisitis detectionis satisfaceret, statutum est ut sub condicionibus chromatographicis supra dictis, efficientia columnae chromatographicae in gel, id est, numerus theoreticus laminarum (N), non minor esset quam 10000, computatus ex cacuminibus normae tripeptidi (Glycina-Glycina-Glycina).
- 4.2 Productio curvarum normalium massae molecularis relativae
- Hae solutiones peptidorum supradictae, cum concentratione massae 1 mg/mL, per adaptationem phasis mobilis praeparatae sunt, deinde per membranam phasis organicae cum pororum magnitudine 0.2 μm~0.5 μm filtratae et in specimen iniectae, deinde chromatogrammata normarum obtenta sunt. Curvae calibrationis massae molecularis relativae et earum aequationes per delineationem logarithmi massae molecularis relativae contra tempus retentionis vel per regressionem linearem obtentae sunt.
4.3 Tractatio exemplaris
In ampulla volumetrica 10 mL accurate pondera 10 mg exempli, adde paulum phasis mobilis, agita ultrasonico per 10 min, ut exemplum plene dissolvatur et misceatur, deinde cum phase mobili ad scalam dilue, deinde per membranam phasis organicae cum pororum magnitudine 0.2 μm ~ 0.5 μm filtra, et filtratum secundum condiciones chromatographicas in A.4.1 analysatum est.
- 5. Computatio distributionis massae molecularis relativae
- Post analysin solutionis exemplaris praeparatae in 4.3 sub condicionibus chromatographicis 4.1, massa molecularis relativa exemplaris et eius ambitus distributionis obtineri possunt substituendo data chromatographica exemplaris in curvam calibrationis 4.2 cum programmate GPC ad data tractanda. Distributio massarum molecularium relativarum peptidorum diversorum calculari potest methodo normalizationis areae cacuminis, secundum formulam: X = A/A total × 100.
- In formula: X - Fractio massae peptidi massae molecularis relativae in peptido totali in exemplo, %;
- A - Area apicis peptidi massae molecularis relativae;
- Summa A — summa arearum apicorum cuiusque peptidi massae molecularis relativae, ad unum locum decimalem computata.
- 6 Repetibilitas
- Differentia absoluta inter duas determinationes independentes sub condicionibus repetibilitatis obtentae non excedere debet 15% mediae arithmeticae duarum determinationum.
- Appendix B: Methodi ad Aminoacida Liberorum Determinanda
- Adoptio normae: Q/320205 KAVN05-2016
- 1.2 Reagentia et materiae
- Acidum aceticum glaciale: analytice purum
- Acidum perchloricum: 0.0500 mol/L
- Indicator: 0.1% indicator crystallini violacei (acidum aceticum glaciale)
- 2. Determinatio aminoacidorum liberorum
Exempla ad 80°C per horam unam siccata sunt.
Exemplum in vase sicco pone ut naturaliter ad temperaturam ambientem refrigescat vel ad temperaturam utilem refrigescat.Pondera circiter 0.1 g exempli (accurate ad 0.001 g) in lagenam conicam siccam 250 mL.Cito ad gradum proximum procede ne exemplum humorem ambientem absorbeat.Adde 25 mL acidi acetici glacialis et bene misce non plus quam quinque min.Adde duas guttas indicatoris crystallini violacei.Titra cum solutione titrationis normali acidi perchlorici 0.0500 mol/L (±0.001) donec solutio a purpureo ad punctum finale mutetur.
Volumen solutionis normae consumptum nota.
- Simul experimentum inanis perage.
- 3. Computatio et eventus
- Quantitas aminoacidi liberi X in reagendo exprimitur ut fractio massae (%) et calculatur secundum formulam: X = C × (V1-V0) × 0.1445/M × 100%, in formula:
- C - Concentratio solutionis acidi perchlorici ordinarii in moles per litrum (mol/L)
- V1 - Volumen adhibitum ad titrationem exemplorum cum solutione acidi perchlorici normalis, in millilitris (mL).
- Vo - Volumen adhibitum ad titrationem inanis cum solutione acidi perchlorici normalis, in millilitris (mL);
M - Massa exempli, in grammis (g).
| 0.1445: Massa media aminoacidorum aequivalentis 1.00 mL solutionis acidi perchlorici normalis [c(HClO4) = 1.000 mol/L]. | 4.2.3 Solutio titrationis cerii sulfatis norma: concentratio c [Ce(SO4)2] = 0.1 mol/L, praeparata secundum GB/T601. | |
| Adoptio normarum: Q/70920556 71-2024 | 1. Principium determinationis (Fe ut exemplum) | Complexus ferri aminoacidorum solubilitatem parvam in aethanolo anhydrico habent, et iones metallici liberi in aethanolo anhydrico solubiles sunt; differentia solubilitatis inter utrumque in aethanolo anhydrico adhibita est ad ratem chelationis complexus ferri aminoacidorum determinandam. |
| In formula: V1 - volumen solutionis normae cerii sulfatis consumptum ad titrationem solutionis probatae, mL; | Ethanol anhydricum; cetera eadem sunt ac in clausula 4.5.2 in GB/T 27983-2011. | 3. Gradus analysis |
| Duo experimenta parallela perage. Ponderanda sunt 0.1g exempli ad 103±2℃ siccati per horam unam, accurate ad 0.0001g, 100mL ethanoli anhydrici adde ut dissolvatur, cola, residuum percolatum cum 100mL ethanoli anhydrici saltem ter ablue, deinde residuum in lagenam conicam 250mL transfere, 10mL solutionis acidi sulfurici adde secundum clausulam 4.5.3 in GB/T27983-2011, et deinde sequentia secundum clausulam 4.5.3 "Calefac ut dissolvatur et deinde refrigerari permitte" in GB/T27983-2011 perage. Simul experimentum inanis perage. | 4. Determinatio totius ferri contenti | 4.1 Principium determinationis idem est ac in clausula 4.4.1 in GB/T 21996-2008. |
4.2. Reagentia et Solutiones
| 4.2.1 Acidum mixtum: Adde 150mL acidi sulfurici et 150mL acidi phosphorici ad 700mL aquae et bene misce. | 4.2.2 Solutio indicatoris natrii diphenylamini sulfonatis: 5g/L, praeparata secundum GB/T603. | 4.2.3 Solutio titrationis cerii sulfatis norma: concentratio c [Ce(SO4)2] = 0.1 mol/L, praeparata secundum GB/T601. | |
| 4.3 Gradus analysis | Duo experimenta parallela perage. Ponderanda est 0.1g exempli, accurate ad 0.20001g, in ampullam conicam 250mL ponenda, addenda sunt 10mL acidi mixti, post dissolutionem, addenda sunt 30ml aquae et 4 guttas solutionis indicatoris natrii dianilini sulfonatis, deinde sequentia secundum clausulam 4.4.2 in GB/T21996-2008 peragenda sunt. Simul experimentum inanis perage. | 4.4 Repraesentatio eventuum | Ferrum totum X1 complexorum ferri aminoacidorum secundum fractionem massae ferri, valor in % expressus, secundum formulam (1) calculatum est: |
| X1 = (V - V₀) × C × M × 10⁻³ × 100 | V₀ - solutio norma cerii sulfatis ad titrationem solutionis inanis consumptæ, mL; | V₀ - solutio norma cerii sulfatis ad titrationem solutionis inanis consumptæ, mL; | C - Concentratio actualis solutionis normae cerii sulfatis, mol/L5. Computatio quantitatis ferri in chelatisFerri contentum X² in chelato, secundum fractionem massae ferri, valore in % expresso, secundum formulam hanc calculatum est: x² = ((V₁-V₂) × C × 0.05585)/m₁ × 100. |
| In formula: V1 - volumen solutionis normae cerii sulfatis consumptum ad titrationem solutionis probatae, mL; | V2 - solutio norma cerii sulfatis consumpta ad titrationem solutionis inanis, mL;nom1 - Massa exempli, g. Mediam arithmeticam eventuum determinationis parallelae ut eventum determinationis sumatur, et differentia absoluta eventuum determinationis parallelae non plus quam 0.3% sit. | 0.05585 - massa ferri ferrosi in grammis expressa, aequivalens 1.00 mL solutioni normali cerii sulfatis C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1 - Massa exempli, g. Mediam arithmeticam eventuum determinationis parallelae ut eventum determinationis sumatur, et differentia absoluta eventuum determinationis parallelae non plus quam 0.3% sit. | 6. Computatio chelationis celeritatisRatio chelationis X3, valor in %) expressus, X3 = X2/X1 × 100.Appendix C: Methodi ad Determinandam Chelationem Zinpro |
Adoptio normae: Q/320205 KAVNO7-2016
1. Reagentia et materiae
a) Acidum aceticum glaciale: analytice purum; b) Acidum perchloricum: 0.0500 mol/L; c) Indicator: 0.1% indicator crystallini violacei (acidum aceticum glaciale)
2. Determinatio aminoacidorum liberorum
2.1 Exempla ad 80°C per horam unam siccata sunt.
2.2 Exemplum in vase sicco pone ut naturaliter ad temperaturam ambientem refrigescat vel ad temperaturam utilem refrigescat.
2.3 Pondera circiter 0.1 g exempli (accurate ad 0.001 g) in ampullam conicam siccam 250 mL.
2.4 Cito ad gradum proximum procede ne exemplum humorem ambientis absorbeat.
2.5 Adde 25 mL acidi acetici glacialis et bene misce non plus quam 5 min.
2.6 Adde duas guttas indicatoris violacei crystallini.
2.7 Titra cum solutione titrationis normali acidi perchlorici 0.0500mol/L (±0.001) donec solutio a purpurea in viridem per 15s mutetur, sine mutatione coloris ut punctum finale.
2.8 Volumen solutionis normalis consumptum nota.
2.9 Experimentum inanis simul perage.
- 3. Computatio et eventus
- Catalana
- Physicochemical parameters
V1 - Volumen adhibitum ad titrationem exemplorum cum solutione acidi perchlorici normalis, in millilitris (mL).
Vo - Volumen adhibitum ad titrationem inanis cum solutione acidi perchlorici normalis, in millilitris (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Inscriptio: Via Qingpu No. 147, Oppidum Shouan, Comitatus Pujiang, Urbs Chengdu, Provincia Sichuan, Sina.
Telephonum: 86-18880477902
Producta
Mineralia vestigialia inorganica
- Mineralia vestigialia organica
- Suahilica
- Servitium ad personam aptatum
- Nexus celeres
Descriptio Societatis
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Preme pro inquisitione | © Iura omnia reservantur - MMX-MMXXXV. | Index situs QUAESTIONES PRAECIPUAE Telephonum |
| Tel. | 86-18880477902 | Javanensis | Epistula electronica |
| 8618880477902 | Sinensis | Francogallica | |
| Bird | Sinensis | Francogallica | Germanica Hispanica |
| Aquatic animals | Iaponicus | Coreana | Arabica Graecum |
| Turcica | Italica | ||
| Ruminant animal g/head day | January 0.75 | Indonesiana Afrikaans Suecica |
Polonica
- Vasconica
- Catalana
- Physicochemical parameters
Hindica
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgarica
- Cebuana
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatica
Batavus
| Application object | Urdu Vietnamica | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitianus | Hausa | Kinyarwanda Hmong Hungarica |
| Piglets and fattening pigs | Igbo | Javanensis | Kannada Khmer Kurdica |
| Kirghizistanus | Latina | ||
| Bird | 300～400 | 45～60 | Macedonica Malaica Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norvegica
- Pashtonica
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbice
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Suahilica
Tajik
Tamilensis
Teluguensis
Thaiensis
| Application object | Urdu Vietnamica | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yoruba | Zuluensis | Kinyarwanda Oriya Turcomanicus |
| Uigurum | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
